pet28a plasmid backbone Search Results


pet28a vector backbone  (New England Biolabs)
99
New England Biolabs pet28a vector backbone
Cloning of hTSHR‐169 in <t>pET28a</t> (+) vector. (A) Schematic representation of the pET28a (+) expression vector‐harboring gene encoding hTSHR‐169 protein. (B) PCR product of recombinant clone (amplicon size 689 bp); M: DNA Marker; NC, negative control; lane 1–5: recombinant clone, replication 1–5. (C) Restriction enzyme analysis of recombinant pET28a‐hTSHR‐169 expression vector. M: DNA Marker; lane 1: undigested pET28a (+) vector; lane 2: digested pET28a (+) vector; lane 3: undigested recombinant pET28a (+) vector; lane 4: digested recombinant pET28a (+) vector with BamHI and XhoI, where 5.32 kb refers to the fragment of pET28a (+) vector (arrowhead) and 411 bp band refers to the hTSHR‐169 cDNA insert (arrow). (D) Alignment between sequences of cDNA insert against hTSHR sequence
Pet28a Vector Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28a vector backbone/product/New England Biolabs
Average 99 stars, based on 1 article reviews
pet28a vector backbone - by Bioz Stars, 2026-05
99/100 stars
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pet28a fln elp  (New England Biolabs)
99
New England Biolabs pet28a fln elp
Cloning of hTSHR‐169 in <t>pET28a</t> (+) vector. (A) Schematic representation of the pET28a (+) expression vector‐harboring gene encoding hTSHR‐169 protein. (B) PCR product of recombinant clone (amplicon size 689 bp); M: DNA Marker; NC, negative control; lane 1–5: recombinant clone, replication 1–5. (C) Restriction enzyme analysis of recombinant pET28a‐hTSHR‐169 expression vector. M: DNA Marker; lane 1: undigested pET28a (+) vector; lane 2: digested pET28a (+) vector; lane 3: undigested recombinant pET28a (+) vector; lane 4: digested recombinant pET28a (+) vector with BamHI and XhoI, where 5.32 kb refers to the fragment of pET28a (+) vector (arrowhead) and 411 bp band refers to the hTSHR‐169 cDNA insert (arrow). (D) Alignment between sequences of cDNA insert against hTSHR sequence
Pet28a Fln Elp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28a fln elp/product/New England Biolabs
Average 99 stars, based on 1 article reviews
pet28a fln elp - by Bioz Stars, 2026-05
99/100 stars
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pet28a backbone  (Addgene inc)
94
Addgene inc pet28a backbone

Pet28a Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28a backbone/product/Addgene inc
Average 94 stars, based on 1 article reviews
pet28a backbone - by Bioz Stars, 2026-05
94/100 stars
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backbone sequence  (Addgene inc)
93
Addgene inc backbone sequence

Backbone Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/backbone sequence/product/Addgene inc
Average 93 stars, based on 1 article reviews
backbone sequence - by Bioz Stars, 2026-05
93/100 stars
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pet28a backbone  (Millipore)
90
Millipore pet28a backbone

Pet28a Backbone, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet28a backbone/product/Millipore
Average 90 stars, based on 1 article reviews
pet28a backbone - by Bioz Stars, 2026-05
90/100 stars
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pet 28a backbone  (Addgene inc)
94
Addgene inc pet 28a backbone

Pet 28a Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet 28a backbone/product/Addgene inc
Average 94 stars, based on 1 article reviews
pet 28a backbone - by Bioz Stars, 2026-05
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backbone plasmid pet28a sumo nup53xl 1 320  (Addgene inc)
92
Addgene inc backbone plasmid pet28a sumo nup53xl 1 320

Backbone Plasmid Pet28a Sumo Nup53xl 1 320, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/backbone plasmid pet28a sumo nup53xl 1 320/product/Addgene inc
Average 92 stars, based on 1 article reviews
backbone plasmid pet28a sumo nup53xl 1 320 - by Bioz Stars, 2026-05
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Image Search Results


Cloning of hTSHR‐169 in pET28a (+) vector. (A) Schematic representation of the pET28a (+) expression vector‐harboring gene encoding hTSHR‐169 protein. (B) PCR product of recombinant clone (amplicon size 689 bp); M: DNA Marker; NC, negative control; lane 1–5: recombinant clone, replication 1–5. (C) Restriction enzyme analysis of recombinant pET28a‐hTSHR‐169 expression vector. M: DNA Marker; lane 1: undigested pET28a (+) vector; lane 2: digested pET28a (+) vector; lane 3: undigested recombinant pET28a (+) vector; lane 4: digested recombinant pET28a (+) vector with BamHI and XhoI, where 5.32 kb refers to the fragment of pET28a (+) vector (arrowhead) and 411 bp band refers to the hTSHR‐169 cDNA insert (arrow). (D) Alignment between sequences of cDNA insert against hTSHR sequence

Journal: Journal of Clinical Laboratory Analysis

Article Title: Comparative performance of ELISA and dot blot assay for TSH‐receptor antibody detection in Graves’ disease

doi: 10.1002/jcla.24288

Figure Lengend Snippet: Cloning of hTSHR‐169 in pET28a (+) vector. (A) Schematic representation of the pET28a (+) expression vector‐harboring gene encoding hTSHR‐169 protein. (B) PCR product of recombinant clone (amplicon size 689 bp); M: DNA Marker; NC, negative control; lane 1–5: recombinant clone, replication 1–5. (C) Restriction enzyme analysis of recombinant pET28a‐hTSHR‐169 expression vector. M: DNA Marker; lane 1: undigested pET28a (+) vector; lane 2: digested pET28a (+) vector; lane 3: undigested recombinant pET28a (+) vector; lane 4: digested recombinant pET28a (+) vector with BamHI and XhoI, where 5.32 kb refers to the fragment of pET28a (+) vector (arrowhead) and 411 bp band refers to the hTSHR‐169 cDNA insert (arrow). (D) Alignment between sequences of cDNA insert against hTSHR sequence

Article Snippet: The hTSHR‐169 fragment and pET28a (+) vector backbone were ligated by T4 DNA ligase enzyme (New England Biolabs) at 16°C for 75 min.

Techniques: Clone Assay, Plasmid Preparation, Expressing, Recombinant, Amplification, Marker, Negative Control, Sequencing

Journal: eLife

Article Title: In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates

doi: 10.7554/eLife.64252

Figure Lengend Snippet:

Article Snippet: The 6xHis-MBP tag was PCR-amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into pET28a backbone with Xba I and Eco RI restriction sites. mGFP-FUS-TIS-Strep-tag II was PCR-amplified from pcDNA-mGFP-FUS-TIS construct with primers mGFP F and TIS RBD-Strep-tag R. The Strep-tag II sequence was incorporated into primer TIS RBD-Strep-tag R. Full-length mGFP-FUS-TIS-Strep-tag II was cloned into pET28a-6xHis-MBP backbone with Nhe I and Eco RI restriction sites.

Techniques: Transfection, Construct, Recombinant, Expressing, Plasmid Preparation, In Vitro, Sequencing, Purification, Mutagenesis, Bradford Protein Assay, Bradford Assay, Protein Quantitation, Software, Strep-tag, Imaging